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. 2020 Apr 16;14(5):845–860. doi: 10.1016/j.stemcr.2020.03.014

Figure 2.

Figure 2

Spatiotemporal Protein Expression of TH Signaling Components

(A) Perfusion-fixed coronal forebrain cryosections were immunostained to visualize DIO3, LAT1, LAT2, MCT8, and MCT10 protein (green) in the SGZ in GFAP+ (magenta)/SOX2+ (blue) NSCs, in MCM2+ (magenta) proliferating cells, in DCX+ (magenta) cells either negative for CR (type 2b progenitors and NBs) or CR+ (blue; INs), and in CB+ (magenta) GCNs. Nuclei were stained with Hoechst 33258 (gray).

(B) MCT8 (green) co-stained with MCM2 (blue) in DCX+ (magenta; arrowhead) but not DCX− (∗) cells. Cell nuclei counterstained by Hoechst 33258 are displayed in gray.

(C) Distribution of DIO3, LAT2, MCT8, and MCT10 (green) over the height of the suprapyramidal blade of the dentate gyrus. Nuclei were stained for Hoechst 33258 (gray) and mature GCNs were identified by CB (magenta). DIO3 and LAT2 protein are asymmetrically distributed with higher signal intensities in areas close to the molecular layer, while MCT8 and MCT0 appear evenly dispersed.