Figure 5.

MCT8 Deficiency Compromises Adult Hippocampal Neurogenesis at 6 Months of Age

Neurogenesis was assessed in 6-month-old males.

(A) Numbers of GFAP+ (cyan)/SOX2+ (yellow) NSCs with a radial process (arrowheads) as well as density of activated KI67+ (magenta; arrow) NSCs.

(B) Proliferation 3 days after EdU injection. Overall KI67+ (cyan; arrows) and KI67+/EdU+ (yellow) cell numbers are shown. Late-stage proliferating cells expressing DCX (magenta; arrowheads) show a higher proliferative capacity.

(C) Density of DCX+ (magenta)/CR− (cyan) type 2b progenitors/NBs (arrowheads) as well as of DCX+/CR+ INs (arrows) with or without EdU (3 dpi; yellow).

(D) Newly formed GCNs (arrowheads) positive for CB (green) and EdU (magenta) were visualized 28 days after EdU injection.

(E) Breeding strategy to generate males harboring the WT or Mct8 KO allele as well as Nestin-CreERT2 and Rfp reporter transgenes. Animals were gavaged for 5 consecutive days at 4 weeks of age and perfused at 6 months of age.

(F) Numbers of GFAP+ (cyan)/SOX2+ (yellow) NSCs (arrows) with a radial process and of RFP+ (in magenta; arrowheads) NSCs were counted and quantified as per mm and percentage of RFP+ cells.

(G) RFP+ (magenta)/CB+ (green) GCNs (arrowheads) were counted and normalized to the number of RFP+ cells. Cell nuclei were stained with Hoechst 33258 (blue). n = 4 (28 dp EdU and Nestin-Cre; Rfp animals) or n = 6 (3 dp EdU injection) mice per genotype. Group means + SEM are shown.∗, p < 0.05; ∗∗, p < 0.01; ∗∗∗, p < 0.001, unpaired two-tailed Student’s t test.