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. 2020 Apr 16;14(5):845–860. doi: 10.1016/j.stemcr.2020.03.014

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© 2020 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Absence of MCT8 in NSCs Compromises Adult Hippocampal Neurogenesis

(A) Mct8fl/+ females were bred with males carrying Nestin-CreERT2 and Rfp reporter alleles to generate Mct8+/y, Nestin-CreERT2, Rfp (control), and Mct8fl/y, Nestin-CreERT2, Rfp (MCT8-NSC KO) littermates. Tamoxifen was given for 5 consecutive days at 4 weeks of age and animals were perfused at 6 months of age.

(B) Number of RFP+ (magenta)/GFAP+ (yellow)/SOX2+ (cyan) NSCs per mm SGZ and their percentage contribution to all RFP+ cells was determined.

(C–F) Relative numbers of RFP+ (magenta; arrowheads)-labeled activated NSCs (KI67+ [yellow]/GFAP+ [cyan]) (C), proliferating cells (KI67+; cyan) and proliferating DCX+ (yellow) cells (D), apoptotic cells (caspase-3+; green) (E), DCX+ (cyan)/CR−(yellow) type 2b progenitors/NBs, and RFP+/DCX+/CR+ INs (arrows) (F).

(G) Ratio of RFP+ (magenta)/CB+ (green) GCNs (arrowheads) over all RFP+ cells. Hoechst 33258-labeled cell nuclei are depicted in blue. n = 5 mice per genotype. Group means + SEM are shown.∗∗, p < 0.01, unpaired two-tailed Student’s t test.