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. 2020 Apr 16;14(5):861–875. doi: 10.1016/j.stemcr.2020.03.019

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© 2020 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

IL-6 Increases PFMP Cell Proliferation and Decreases the Proliferation of NSCs and Other Progenitors In Vitro

SVZ neurospheres produced from P4 mice were treated with 5 ng/mL rmIL-6. After 10 h, 10 μM EdU was added and 2 h later the cells were analyzed by flow cytometry using a multi-marker panel comprised of anti-CD133/LEX/NG2/CD140a followed by the Click-It reaction for EdU.

(A) Schematic of in vitro analysis of IL-6 effects on EdU incorporation in SVZ NSPs.

(B) Debris and dead cells were excluded using LIVE/DEAD blue.

(C and D) (C) After gating on CD140⁺/NG2⁺ cells this subset was separated based on intensity of CD133 and LeX (D).

Positively EdU stained cells were gated based on isotype control for PFMP (E), BNAP (F), GRP2 (G), GRP3 (H), MP1 (I) and NSC (J). Data are representative of two independent experiments conducted with triplicate technical replicates.