TABLE 1.
qPCR primers for candidate reference genes and characteristics of PCR amplification in O. communa.
| Gene name | Accession number | Primer sequence (5′–3′) | Product length (bp) | Tm (°C) | E (%) | R2 |
| RPL4 | MN641110 | F: TGTGGTAATGCTGTGGTAT | 104 | 60 | 99.14 | 0.998 |
| R: TCTAGCACTGCATGAACA | ||||||
| RPS18 | MN641111 | F: TCCGATGGCTGTATGTTAC | 126 | 60 | 98.145 | 0.998 |
| R: GCTGTTGGGAGGAGTTAT | ||||||
| ACT1 | MN641112 | F: CAGTGGTGATGGTGTTAC | 148 | 60 | 97.263 | 0.999 |
| R: CTCAGGAGGTATGCCAAT | ||||||
| ACT2 | MN641113 | F: GTTGCTCAGAGGTTATGC | 149 | 60 | 105.63 | 0.997 |
| R: GGTAAGGTGTAAGGTTCCA | ||||||
| ARF1 | MN641114 | F: GAGATGCCGTGTTGTTGA | 99 | 60 | 102.166 | 0.997 |
| R: TGCGTAGCGAATGAAGAC | ||||||
| ARF4 | MN641115 | F: GTGTAGATATGGAGCGAATG | 98 | 60 | 103.345 | 1 |
| R: GTTAGCGAGCACTAGAATC | ||||||
| SDH | MN641116 | F: ACTTCCGCTCATACTTGT | 149 | 60 | 98.306 | 0.999 |
| R: CCTCCTTCACCTCTACATC | ||||||
| GADPH | MN641117 | F: TGCTTCAACCGACACATT | 136 | 60 | 102.673 | 0.999 |
| R: CGACCAATACGACCGAAT | ||||||
| EF1α | MN641118 | F: AGAACAATCCACCAAGAGG | 98 | 60 | 101.81 | 0.996 |
| R: GTCCAACACAGGCGTATA | ||||||
| βTUBC | MN641119 | F: TCAGAGGTAGTCCTTCCA | 103 | 60 | 100.58 | 0.996 |
| R: CAATTATCGCAGTTCTCCG | ||||||
| COX | MN641121 | F: GCTGAACACAGTTATTCTGA | 150 | 60 | 98.533 | 0.998 |
| R: AGAGGCTCTATCTTGAAGT | ||||||
| RPL19 | MN641120 | F: AAGGAAGGCATTGTGGAT | 94 | 60 | 97.046 | 0.997 |
| R: GACGCAAATCTCGCATAC |