Figure 3.

Effect of heat stress on intestinal HSP expression in modern broilers and wild JF birds. The relative expression of HSP60, HSP70, and HSP90 was determined in the duodenum (A-C), jejunum (D-F), and ileum (G-I) using real-time quantitative PCR and 2^−ΔΔCt method (30). Data are mean ± SEM (n = 8/group). Different letters indicate significant difference at P < 0.05. ACRB, Athens Canadian Random Bred; HS, heat stress; JF, jungle fowl; MRB, modern random bred; RH, relative humidity; TN, thermoneutral.