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. 2020 May 7;7:249. doi: 10.3389/fvets.2020.00249

Figure 6.

Figure 6

Effect of heat stress on intestinal tight junction expression in modern broilers and wild JF birds. The relative expression of OCLN (A), ZO-1 (B), ZO-2 (C), ZO-3 (D), JAMA (E), GJA1 (F), PATJ (G), CDH1 (H), Cx45 (I), VIL1 (J), LCN2 (K), and CALPR (L) was determined in the ileum using real-time quantitative PCR and 2−ΔΔCt method (30). Data are mean ± SEM (n = 8/group). Different letters indicate significant difference at P < 0.05. ACRB, Athens Canadian Random Bred; CALPR, calprotectin; CDH1, cadherin 1; Cx45, connexin 45; GJA1, gap junction protein alpha 1; HS, heat stress; JAMA, junctional adhesion molecule A; JF, jungle fowl; LCN2, lipocalin 2; MRB, modern random bred; OCLN, occludin; PATJ, PALS1-associated tight junction protein; RH, relative humidity; TN, thermoneutral; VIL1, villin 1; ZO-1/2/3, zonula occludens.