Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 May 7;14:401. doi: 10.3389/fnins.2020.00401

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2020 Wali, Liyanage, Blair, Sutharsan, Park, Mackay-Sim and Sue.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Generation and characterization of iPS cell lines and differentiation into neurons. (A) Timeline of reprogramming skin fibroblasts to iPS cells. (B–E) Expression of pluripotency markers SSEA-4 and Sox2 in patient and control iPS cells and group means (+/− SEM). (D) Flowcytometry based analysis of control and patient cells. (F,G) Embryoid body cells expressing three germ layer markers: β III-tubulin (ectoderm), brachyury (mesoderm), GATA4 (endoderm). (H) Timeline of iPS cell differentiation to forebrain neurons. Brain-derived neurotrophic factor (B), ascorbic acid (A), glial cell-derived neurotrophic factor (G), DAPT (D), and dibutyryl cAMP (C). (I) Expression of neuronal differentiation markers and quantification of neurons immuno-labeled for β III-tubulin and FOXG1 expression. Scale bar: 50 microns.