Fig. 3.

Enhanced MMP-2 and MMP-9 activity is mediated by the NF-κB pathway in the AGEs-treated NRK-52E cells. (A) Immunodetection of the NF-κB subunit p65 in the nucleus fraction of AGEs-treated NRK-52E cells followed by separation on 10% SDS–polyacrylamide gels. (B) Activation of the NF-κB promoter at the binding site in AGEs-treated and untreated NRK-52E cells was analyzed using the luciferase reporter assay. Translocation of NF-κB as a transcription factor from the cytoplasm to the nucleus in NRK-52E cells. Effect of treatment with NF-κB inhibitor (BAY11-7082) on the expression of (C) MMP-2 and (D) MMP-9 were evaluated using qRT-PCR