Fig. 4.

Detection of MMP-2 and MMP-9 expression after siRAGE transfection. Effects of RAGE knockdown by siRNA transfection in NRK-52E cells. NRK-52E were transfected with siRAGE (100 nM) using Lipofectamine 2000 transfection reagents and then treated with AGEs (100 µg/mL) for 24 h. Total mRNA was extracted and MMP-2, MMP-9, and β-actin expression was determined using qRT-PCR. (A) Downregulation of RAGE mRNA expression. (B) MMP-2 and (C) MMP-9 expression was enhanced by siRAGE-transfection of AGEs-treated NRK-52E cells