Figure 1.

Elevated Mitochondrial/ER Activities and MFN Expression during Spermatogonial Development

(A and B) ROS (A) and cellular Ca²⁺ levels (B) were measured in CD90+/KIT− undifferentiated spermatogonia and KIT+ differentiating spermatogonia collected from wild-type P12 testes by flow cytometry.

(C) Real-time RT-PCR analyses on the expression of ER chaperons in sorted CD9+/KIT− undifferentiated spermatogonia and KIT+ differentiating population from wild-type P12 testes.

(D) Protein levels of MFN1 and MFN2 were measured by western blots in testes collected at various time points during spermatogenesis.

(E) Real-time RT-PCR analyses on Mfn1 and Mfn2 expression in sorted CD9+/KIT− and KIT+ cells from wild-type P12 testes.

(A–C and E) Data are presented as mean ± 1 SEM from three or more biological replicates. Fold changes or expression levels were compared with mean value of CD90+/KIT− or CD9+/KIT− cells. ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001; N.S., no statistical significance.