Figure 3.

MFN Deficiency Leads to Apoptosis in Differentiating Spermatogonia and Spermatocytes

(A) IHF with PCNA and DDX4 co-staining on Mfn2^f/+ and Mfn2^f/f; Ddx4-Cre testes at P7 and P14.

(B) Percentage of apoptotic cells was analyzed by flow cytometry with FITC-Annexin V and PI co-staining on CD9+/KIT− undifferentiated spermatogonia and KIT+ cells collected from testes of P12 mice. Data represent mean ± 1 SEM from at least four littermate pairs. ^∗p < 0.05, ^∗∗p < 0.01; N.S., no statistical significance.

See also Figure S2.