Figure 4.

Induction of Endogenous PAX7 Expression Is Sustained after Multiple Passages and Dox Withdrawal

(A) Representative immunostaining of PAX7 and MHC in differentiated iPSCs after four passages in the presence of dox. Scale bar, 200 μm.

(B) Representative immunostaining of PAX7 and myosin heavy chain (MHC) after inducing differentiation by dox withdrawal for 7 days. Scale bar, 200 μm.

(C) Quantification of PAX7⁺ nuclei after no passages and after an average of four additional passages with dox or after dox withdrawal (mean ± SEM, n = 3 independent experiments).

(D) Representative immunostaining of the FLAG epitope for ^VP64dCas9^VP64 after dox withdrawal for 7 days. Scale bars, 100 μm.