Figure 6.

Identification of Endogenous versus Exogenous PAX7-Induced Global Transcriptional Changes

(A) Expression heatmap of sample-to-sample distances in the matrix using the whole gene-expression profiles among the four groups and their replicates.

(B) Heatmap showing differential expression of top 200 variable genes between all four groups after filtering genes with low read counts. The color bar indicates Z score.

(C) Venn diagram of genes overexpressed in each group relative to gRNA only (fold change >2 and padj < 0.05).

(D) GO biological process terms of shared genes between the three groups derived from the Venn diagram in (C). Term list was generated using Enrichr; p values were computed using Fisher’s exact test.

(E) Expression profiles of select pre-myogenic, myogenic, and satellite cell marker genes from RNA-seq data (mean ± SEM, n = 3 independent replicates). TPM, transcripts per million.