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. 2020 May 7;8:287. doi: 10.3389/fcell.2020.00287

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Copyright © 2020 Quan, Guo, Liu, Yang, Bai, Wang, Cai, Han, Lv, Ding, Billadeau, Lou, Bellusci, Li and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Pharmacological FGFR inhibition impairs pancreatic cancer stemness. (A) Sphere formation assay using L3.6 cells with different doses of FGFR inhibitors (AZD4547 and Dovitinib) and sphere number quantification from three independent experiments. scale bar: 200 μm. (B,C) Determination in L3.6 cells of FGF2-induced FGFR phosphorylation by western blot in the presence of AZD4547 or Dovitinib. FGFR1 and alpha-Tubulin are used as loading controls. Numbers below the blots are quantifications for three independent experiments. (D) Cell survival rate after 72 h of treatment with different doses of AZD4547 or DMSO control. (E) Determination by western blot of L3.6 cells of cleaved Caspase 3 in the presence of AZD4547. (F) Expression of stemness markers CD24, CD44, and CD133 by qPCR in PDAC with and without AZD4547 treatment. *p ≤ 0.05, **p ≤ 0.01.