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. 2020 May 7;8:287. doi: 10.3389/fcell.2020.00287

FIGURE 2.

FIGURE 2

Genetic silencing of FGFR expression leads to reduced stemness in vitro and tumor formation in vivo. (A,B) Expression of FGFRs by qPCR and western blot in L3.6 cells upon silencing specific FGFRs. Numbers below the blots are quantifications for three independent experiments. (C) Sphere formation assay in L3.6 cells following specific FGFR knockdown and quantification of sphere numbers for three independent experiments. Scale bar: 200 μmm. (D) FGFR1 and FGFR2 protein expression by western blot in several pancreatic cancer cell lines. (E) Tumor formation rate 3 weeks following the subcutaneous inoculation of different numbers of L3.6 cells to nude mice. (F) Expression of stemness markers CD24, CD44, and CD133 by qPCR in L3.6 cells upon silencing of FGFR1 or FGFR2. *p ≤ 0.05, **p ≤ 0.01.