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. 2020 May 7;8:287. doi: 10.3389/fcell.2020.00287

FIGURE 3.

FIGURE 3

SOX2 expression in L3.6 cells correlates with stemness and silencing of SOX2 expression leads to decreased spheroid formation. (A) Monolayer and spheroid L3.6 cells were stained with DAPI, SOX2 and CD24. Scale bar: 20 μm. (B) Expression of SOX2 by western blot in monolayer and spheroid L3.6 cells. Numbers below the blots are quantifications for three independent experiments. (C) Sphere formation assay using SOX2 knockdown in L3.6 cell lines compared with scramble control. Scale bar: 200 μm. (D) Western blot was performed to detect the expression of SOX2 in SOX2 knockdown L3.6 cells compared to scramble control. Numbers below the blots are quantifications for three independent experiments. (E) Quantification of sphere diameter in scramble control vs. SOX2 knockdown (each spot represents one sphere). (F) Expression of stemness markers CD24, CD44 and CD133 by qPCR in L3.6 cells upon silencing of SOX2. **p ≤ 0.01, ***p ≤ 0.001.