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. 2020 May 7;8:287. doi: 10.3389/fcell.2020.00287

FIGURE 6.

FIGURE 6

FGFR regulates SOX2 mainly through AKT. (A) Western blot analysis of key FGFR downstream pathways in L3.6 cells treated with different doses of AZD4547 together with FGF2 (10 ng/ml) for 12 h. (B) Sphere formation assay using L3.6 cells treated with different doses of MK2206 (AKT inhibitor) and LLL12 (STA3 inhibitor). Scale bar: 200 μm. (C) Corresponding sphere number quantification for three independent experiments. (D) Western blot analysis of pathway inhibition efficiency of MK2206 and LLL12 for 24 h. Numbers below the blots are quantifications for three independent experiments. (E) Western blot was performed to quantify SOX2 expression levels upon MK2206 (2 μM) treatment in L3.6 cells at indicated time points. Numbers below the blots are quantifications for three independent experiments. (F) SOX2 detection in cytoplasmic and nuclear fractions upon transfection with AKT-WT or AKT-KD in HEK293. (G) SOX2 detection in cytoplasmic and nuclear fractions upon MK2206 (2 μM) treatment at different time points. (H) Western blot was carried out to quantify SOX2 expression level upon treating with CHX (50 μg/ml) with and without MK2206 (2 μM) in L3.6 cells at different time points. (I) Corresponding quantification for three independent experiments. *p ≤ 0.05, **p ≤ 0.01.