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. 2020 May 7;11:447. doi: 10.3389/fpls.2020.00447

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Copyright © 2020 Skowrońska, Mariańska, Ulaszewski, Tomkowiak, Nawracała and Kwiatek.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Amplification products of PCR reactions of (A) wheat accessions (pollen donors) and (C) F₁ hybrids with csLV34 marker linked to Lr34 (= Yr18/Sr57/Pm38) locus and (B) wheat accessions and (D) F₁ hybrids with Xgwm44 marker linked to Lr46 (= Yr29/Sr58/Pm39). Lr34 or Lr46+ indicate resistant alleles; Lr34- or Lr46– indicate susceptible alleles. Genomic in situ hybridization preformed on mitotic chromosomes of plants derived through cross-hybridization between triticale and wheat: (E) chromatin (DAPI), (F) D-genome chromosmes (Atto-488, green), (G) R-genome chromosomes (Atto-550, red), (H) merged.