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. 2020 May 7;11:803. doi: 10.3389/fimmu.2020.00803

FIGURE 6.

FIGURE 6

H56 uptake by splenic cells following parenteral or airway mucosal prime or parenteral prime – airway mucosal pull immunization with H56/CAF01. BALB/c mice were immunized with Alexa Fluor® 647-labeled H56/DiR-labeled CAF01 via i.m. or i.pulmon. or i.m. – i.pulmon. routes, and the H56+ uptake by spleen cells was assessed by flow cytometry 3, 24, and 72 h post-immunization. Numbers of (A) neutrophils (Ly6G+), (B) pDCs (CD11c+Ly6C+F4/80– CD11b– ), (C) inflammatory monocytes (Ly6C+CD11b+), (D) moDCs (CD11c+F4/80+CD11b+CD64+), (E) CD8α+ DCs (CD11c+CD11b– CD8α +), (F) CD11b+ DCs (CD11c+CD11b+), (G) B cells (CD19+), (H) macrophages (F4/80+CD11b+), and (I) eosinophils (SiglecF+) in the spleen. (J) Fraction of H56+ cells in the spleen at 3, 24, and 72 h post-immunization. Data points represent n = 2, and they display mean values ± SEM. *p < 0.05, **p < 0.01, ****p < 0.0001 vs. i.m. immunization via two-way ANOVA with Tukey’s post-test.