FIGURE 1.
Sorting of chromosomes and Y chromosome data following MinION sequencing. (A) Flow Karyotyping of the RAW264.7 genome. Chromosomes were dyed with PI and Hoechst33342. Y chromosomes were collected from the circled area. (B) Coverage of chromosomes mapped using BWA-MEM. The Y chromosome was enriched with 16.8x coverage. (C–H) The Y chromosome sequencing data from individual experiments. (C) Histogram of read length. (D) Boxplot of read length. (E) Number of reads. (F) Mean length. Error bars denote standard error (SE). (G) N50 length. (H) Total length.