FIGURE 2.

The reads obtained through MinION sequencing were assembled using the Flye assembler. The generated contigs were corrected for error using Racon to obtain the consensus sequences. The polished contigs were used as query sequences and mapped to the reference genome (GRCm38.p6) using BWA-MEM. The alignment results were visualized using D-GENIES. Dot plots were generated to illustrate the pairing between the sequences of the Y chromosome and contigs. (A) The polished contigs were compared with the whole sequence of the reference Y chromosome: 1−91,744,698 bp. (B) The polished contigs were compared with the reference Y chromosome: 1–4,000,000 bp. (C) The sequences of the raw reads with the MinION sequencing were compared with the reference Y chromosome: 1–4,000,000 bp.