TABLE 3.

Mitotypes of hybrids and glycerol utilization as a carbon source.

					Allels of			Growth on glycerol at
					COX1
Category of nuclear nuclear genome1	Category of mitotypes2	Strains	Whole-genome RFLP3	ATP6	COX1 intron4	COX25	COX3	25 °C	37 °C
Parental strains
	C	10-170	C	C	C	C	C	+	+
	U	10-522	U	U	U	U	U	+	−
Hybrids
Method 1
I	C	a2, a4, a5, a6, a7, a9, a10	C					+	+
	I-r1	a1	R1	C	C	Rc1	C	+	+
	I-r2	a3	C					+	+
			R7	U	−	Rc2	U	+	+
	I-r3	a8	R2	U	C	Rc1	U	+	-
Method 2
II	II-r1	A27	R3	U	C	C	U	-	-
III	C	A12, A13, A14, A16, A17, A18, A19, A22, A25, A28, A30, A34	C					+	+
	U	A5	U					+	+
	III-r1	A37	R4	C	C	U	U	+	(+)
	III-r2	A2	R4	C	C	U	U	+	+
	III-r3	A8	R5	C	C	U	U	+	+
	III-r4	A15	R6	C	C	U	U	+	+
	III-r5	A33	R5	C	C	U	U	+	−
	III-r6	A35	R7	U	C	U	U	+	−
	III-r7	A36	R8	C	C	U	U	+	+
	III-r8	A39, A40	R7	U	Ri1	U	U	+	-
IV	C	A7, A10, A20, A23, A26, A31	C					+	+
	IV-r1	A6, A38	R6	C	Ri2	U	U	+	+
	IV-r2	A21	R2	U	C	U	U	+	−
	IV-r3	A24	R2	U	C	U	U	+	−
V	C	A1, A9, A11	C					+	+
VI	C	A29, A32	C					+	+
	U	A3	U					+	+
	VI-r1	A4	R7	U	C	U	U	+	−

¹For description of categories, see Table 1. ²Based on all sets of results shown in the table. ³RFLP pattern types (for examples, see Figure 3): C, S. cerevisiae parental pattern; U, S. uvarum parental pattern. Banding patterns (bands larger than 700 bp, starting the order with the largest band): C: pqrstuvw U: ABCDEF R1: pqrs.uvw R2:..r..A.... ABCD.F 1 and 2 (new bands)R3: r R4: ..r R5: ..r..u. R6: .qrs.uvw ABCDEF ABCDEF ABCDEF A 1 and 2 (new bands) 2 (new band) 2 (new band) R7:. qr..u. R8: ABC.EF ABCD.F 2 (new band) 2 (new band)⁴Ri1 and Ri2 are recombinant COX1 patterns. ⁵Rc1 and Rc2 are recombinant COX2 patterns.