Figure 4.

Temporal Shift of Inhibition Supports Reversal of DSGC’s Directional Preference

(A) An example DSGC directional tuning to drifting gratings (shown on the left) in DA conditions (left) and following RVS (right). RVS is illustrated in the center. Traces are examples of 1-s recordings. Polar plots represent the mean response (spike count during 3 s), and thin lines show the responses in each repetition. Arrows represent the normalized vectorial summation (with outer ring equal to 1).

(B) Mean voltage clamp recordings of four example DSGCs in response to gratings moving in the PD (blue, cyan) and ND (red, orange) in DA conditions (left) and following RVS (right). Horizontal lines represent the detected response phases, which determine time of response onset (vertical continuous and dashed lines for excitation [E] and inhibition [I], respectively).

(C) Cumulative current during PD and ND motion of inhibitory and excitatory average normalized waveforms (mean ± SEM). Vertical lines denote the time of 50% maximal value (PD: blue and cyan, and ND: red and orange, for DA conditions and following RVS, respectively).

(D) DSI of inhibition in DA conditions and following RVS.

(E) Time difference between excitatory and inhibitory response onset during PD and ND motion in DA conditions and following RVS.

For (D) and (E), group means and SD are indicated on the side by circles and error bars; dashed lines connect values of the cells depicted in (B). Asterisks indicate statistical significance (*p<0.05; **p < 0.005).