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. 2020 Feb 13;9(5):e1010. doi: 10.1002/mbo3.1010

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© 2020 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Production of metallothionein fused to various encapsulation peptides in the presence and absence of BMCs. TEM analysis of cells producing metallothionein tagged with the D18, L20, and P18 encapsulation peptides in the absence (top row) and presence (second row) of PduA‐U BMCs. TEM of isolated BMCs from the strains in row 2 is shown in row 3. The bottom row shows TEM tomography of sections cells from row 2—see Video S1 at https://doi.org/10.5281/zenodo.3611413 for full tomography data. All scale bars are 200 nm