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. 2020 Mar 6;9(5):e964. doi: 10.1002/mbo3.964

Table 3.

Overview on the impact of filtering and decontamination on the number of retained ASVs and samples in this study. For details on each of the steps, please refer to the materials and methods section

Step #ASVs #samples
Unfiltered 4,309 152
Decontamination with “decontam” prevalence method 4,275 152
Remove phyla with only one representative 4,248 152
Removal of ASVs present in only one sample 1,016 152
Removal of samples with very low read count (<3,500) 1,016 126
Rarefy to even depth 983 126
Remove all taxa present in sequenced laboratory reagents and equipment 912 126