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. 2020 Apr 1;9(5):e1017. doi: 10.1002/mbo3.1017

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© 2020 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Evaluation of the persistence frequency by IFC evaluation, after 24 hr p.i. at an MOI of 100:1, external and adherent bacteria were removed by lysostaphin treatment. The MG‐63 cells infected with the different strains were stained with the membrane‐impermeable fluorochrome VBFL and analyzed on a flow cytometer (Amnis FlowSight Millipore), acquiring 10,000 events per sample. (a) The graph shows the distribution of spots corresponding to the category assigned by the Spot Counting, for each clone. (b) The graph shows the percentage of full versus empty cells, recovered from spot counting. The graph reports the percentage of spots for cell ± SD for each strain for three different experiments. Statistical significative p‐value ≤ .05*; highly significative ≤ .01**; extremely significative ≤ .001***