Figure 3.

Anti‐TIGIT treatments reduced the proportion of CD4⁺ Tregs but had no effect on CD4⁺ and CD8⁺ T cells and NK cells. Mice (n = 6) were injected i.p. with 1 × 106 ID8 cells ten days before receiving treatments. OC mice were injected thrice in 4‐d intervals with 100 μg of isotype control or anti‐TIGIT mAb. Seven days after this, splenic and ascites cells were collected by Ficoll‐Paque density gradient centrifugation and were stained with specific antibodies; the proportions of CD4⁺ T cells (A), CD8⁺ T cells (B), NK cells (C), and CD4⁺ Tregs (D) were assessed by flow cytometry. Shown are the means ± standard deviation