Figure 5.

On-DNA tool compounds were spiked into a DEL of 7.6 million members at the same concentration as individual library members and then tested with the new selection method. After selection, 6.3 million compounds were detected by sequencing from the magnetic beads, and 6.2 million compounds were detected by sequencing from the PhyTip affinity resin. Some library molecules were detected in the presence and absence of 3CP, indicating background binding to the affinity matrices. Enrichment of the tool compounds was significantly above the diagonal line from background binding, indicating that these were specifically enriched by binding to 3CP.