Figure 2.

Cellular copper uptake after treatment with CuO NP, CuO MP or CuCl₂ in the presence or absence of (A) OH-dyn and (B) bafA1. Copper content was determined in the soluble fraction of BEAS-2B cells after 8 h treatment, with the different copper compounds co-treated with or without OH-dyn, or after 24 h co-treated with or without bafA1, respectively, via GF-AAS. Shown are the mean values of three independent experiments + SD. Statistically significant different from the respective copper compound treatment: * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001 (T-test). 50 µg/mL CuO are equal to 630 µM Cu²⁺.