Table 2.

Repeatability (instrumental precision) and intra-day and inter-day precision data of the LC-ESI-MS/MS method for determination of flavonoid aglycones.

Analyte	Nominal Concentration (ng/mL)	Repeatability (% CV) (n = 10)	Precision
			Inter-day		Intra-day
			Measured Concentration (ng/mL)	CV (%)	Measured Concentration (ng/mL)	CV (%)
Taxifolin	50	2.7	52	4.0	51.5	3.0
	1000	2.0	1003	0.3	997	0.3
	5000	2.3	4870	2.6	4950	1.0
Myricetin	11	2.0	10.59	3.7	10.81	1.7
	1000	1.0	953	4.7	1004	0.4
	3600	3.2	3450	4.2	3488	3.1
Morin	10	2.2	10.5	5.0	10.2	2.0
	1000	4.7	1042	4.2	1022	2.2
	5000	4.0	4862	2.8	4750	5.0
Eriodictyol	15	3.0	14.3	4.7	15.1	0.7
	1000	3.2	974	2.6	982	1.8
	5000	1.8	4766	4.7	5012	0.2
Luteolin	40	2.5	38	5.0	39.2	2.0
	1000	4.8	988	1.2	989	1.1
	4000	4.1	3850	3.8	3886	2.9
Quercetin	30	2.8	29.8	0.7	28.9	3.7
	500	2.7	489	2.2	475	5.0
	3000	2.2	2876	4.1	2995	0.2
3-O-Methylquercetin	15	1.9	14.8	1.3	15.2	1.3
	500	1.7	496	0.8	485	3.0
	3700	1.7	3665	0.9	3710	0.3
Apigenin	12	3.9	12	0.0	11.9	0.8
	1000	3.7	954	4.6	996	0.4
	6000	1.2	5800	3.3	5870	2.2
Naringenin	33	0.5	32.2	2.4	33.5	1.5
	500	2.2	489	2.2	515	3.0
	3000	3.5	3100	3.3	3150	5.0
Kaempferol	33	1.9	33.5	1.5	33.6	1.8
	3000	0.1	2850	5.0	3100	3.3
	20,000	2.0	19,950	0.3	19000	5.0
Isorhamnetin	40	1.7	39.2	2.0	40.5	1.3
	3000	4.5	2890	3.7	2900	3.3
	60,000	2.0	57,000	5.0	61,000	1.7
Isokaempferide(3-O-Methylkaempferol)	250	4.6	240	4.0	242	3.2
	3000	1.2	3050	1.7	3100	3.3
	10,000	1.0	9800	2.0	9560	4.4
Rhamnetin	5	1.3	4.9	2.0	5.1	2.0
	200	2.1	198	1.0	205	2.5
	625	1.9	620	0.8	630	0.8
Chrysin	42	3.4	41	2.4	44	4.8
	500	2.7	498	0.4	480	4.0
	2500	2.1	2420	3.2	2600	4.0
Sakuranetin	70	4.3	68	2.9	69	1.4
	1000	4.1	1050	5.0	1020	2.0
	7000	0.9	6800	2.9	6700	4.3
Prunetin	200	3.8	195	2.5	205	2.5
	3000	4.1	2900	3.3	3050	1.7
	20,000	4.8	19,000	5.0	20,500	2.5
Rhamnazin	70	0.2	69	1.4	72	2.9
	3000	3.5	2850	5.0	3050	1.7
	7000	0.3	6800	2.9	7100	1.4