Table 2.
Determination of NLC-associated MTX when dispersions of MTX-NLCs 1 were submitted to ultrafiltration in buffer and acetonitrile-buffer media.
| Ultrafiltration Media | Time (min) | Total MTX Upper Compartment (µg) | Free MTX Upper Compartment (µg) | MTX Associated to NLCs (µg) | Encapsulation Efficiency (%) |
|---|---|---|---|---|---|
| Buffer 2 | 5 | 12.46 ± 0.09 | 11.50 ± 0.07 | 0.96 ± 0.02 | 3.5 ± 0.1 |
| Buffer 2 | 30 | 3.48 ± 0.01 | 3.28 ± 0.09 | 0.20 ± 0.09 | 0.7 ± 0.3 |
| 10% (v/v) acetonitrile | 5 | 4.6 ± 0.1 | 4.22 ± 0.01 | 0.34 ± 0.01 | 1.24 ± 0.04 |
1 Dispersions (2 mL) containing 50 µL of MTX-NLCs (corresponding to 26 mg mL−1); 2 potassium phosphate buffer (pH 7.0; 0.1 M).