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. 2020 Apr 23;25(8):1960. doi: 10.3390/molecules25081960

Figure 1.

Figure 1

Histamine treatment induced cervical cancer growth in vivo and in vitro. HeLa cells were injected subcutaneously into the lower right side of female BALB/c nude mice. After 6 days, mice were randomly divided into three groups: control, HeLa (no histamine), HA + HeLa (1 mg/kg). Animals were intraperitoneally injected with histamine or PBS (same volume as histamine treatment) every 3 days. (A) HeLa cell proliferation after treatment with different concentrations of histamine (1, 5, 10, 50, 100 ng/mL). HeLa cells were incubated with histamine for 48 h. (B) Average tumor volume was measured every 3 days. (C) Tumor weights were measured at the end of treatment. (D) ELISA analysis for serum VEGF levels. (E) ELISA analysis for serum TNF-α levels. The serum used in (D,E) was obtained by the orbital sinus of mice. Histamine promoted cervical cancer growth according to the tumor growth and tumor weight. Data are presented as the mean ± SEM of at least three independent experiments. * p < 0.05 versus the control group, # p < 0.05 versus the HeLa group.