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. 2020 Apr 23;25(8):1960. doi: 10.3390/molecules25081960

Figure 3.

Figure 3

Histamine induced apoptosis via inhibition of the PI3K/Akt/mTOR signaling pathway in HeLa cells. The PI3K/Akt/mTOR signaling pathway was activated by the treatment of histamine. (A) Immunohistochemistry detection of PI3K, Akt, mTOR and Bax expression level in the cervical tissue of the control group and HeLa group. (B) Western blot analysis of PI3K, Akt, mTOR and Bax expression level from respective tumor tissues. The antibodies used in this experiment were PI3K (ABP52199), Akt (ABM40276), mTOR (ABP51866) and Bax (ABP55948). (C) Western blot analysis of p-PI3K and p-AKT expression level from respective tumor tissues. The antibodies used in this experiment were p-PI3K(ab182651) and p-Akt(ab81283). The locus of p-PI3K and p-Akt was Y607 and S473 respectively. The results are expressed as a percentage of control, which is set at 100%. Data are presented as the mean ± SEM of at least three independent experiments. * p < 0.05 versus the control group, # p < 0.05 versus the HeLa group.