(A) Western blot analysis of PI3K, Akt, mTOR and Bax expression level from respective tumor tissues. The antibodies used in this experiment were PI3K (ABP52199), Akt (ABM40276), mTOR (ABP51866) and Bax (ABP55948). (B) Western blot analysis of p-PI3K and p-Akt expression levels from respective tumor tissues. The antibodies used in this experiment were p-PI3K(ab182651) and p-Akt(ab81283). The locus of p-PI3K and p-Akt was Y607 and S473 respectively. The results are expressed as a percentage of control, which is set at 100%. Data are presented as the mean ± SEM of at least three independent experiments. * p < 0.05 versus the control group, # p < 0.05 versus the HA group.