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Two-liquid phase shake flask fermentations with E. coli BL21 (DE3) pJBEI-6410 in M9 minimal medium with either 0.5% w/v glucose (closed symbols) or glycerol (open symbols) as the sole carbon source. (A) Limonene concentrations (▲, △) in the organic phase and carbon source (■, □) concentrations were determined at regular intervals. (B) Carbon specific limonene yields after 26 h of cultivation. The error bars relate to biological duplicates.
