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. 2020 May 4;14:1705–1716. doi: 10.2147/DDDT.S235969

Figure 3.

Figure 3

CGA enhances lysosomal activity in SH-SY5Y cell via the mTOR/TFEB signaling pathway.

Notes: (A) Lysosomes were labeled with LTR and observed by fluorescence microscopy (Scale bar, 10 µm). (B) Representative Western blot imagines of cathepsin D (24 KDa), p70s6k (59 KDa), P-p70s6k (59 KDa), mTOR (298 KDa) and p-mTOR (298 KDa) in SH-SY5Y cells (left panel); β-actin (43 KDa) were used as a control for protein loading. The right panel shows the relative optical density values of cathepsin D, p70s6k, P-p70s6k, mTOR and p-mTOR between the experimental groups. (C) Immunofluorescence staining of TFEB (red) was visualized with confocal microscopy. The nucleus was stained with DAPI (Scale bar, 50 µm). (D) Representative Western blot imagines of TFEB (65 KDa) indicating nuclear and total protein (left panel); Lamin A/C (74/63 KDa) or β-actin (43 KDa) was used as a control for protein loading. The right panel shows the relative optical density values of TFEB between the experimental groups. Data are expressed as the mean ± s.e.m for five samples per treatment group. *P<0.05, **P<0.01, ***P<0.001 compared with no Aβ25-35 treatment group; #P<0.05, ##P<0.01, ###P<0.01 compared with Aβ25-35 treatment group.