Figure 3.

Evaluation of Cuphen and IONPs cytotoxic activity towards human (MNT-1) and murine (B16F10) melanoma cell lines. (a) Cuphen chemical structure; (b) cell viability after 24 h incubation with different Cuphen concentrations (0.5 to 7 µM); (c) Cuphen half-inhibitory concentration (IC₅₀) values; (d) cell viability after 24 h incubation with IONPs at a concentration ranging from 1 to 7.5 mg/mL; (e) cell viability after a 24 h incubation period with Cuphen (1 and 5 µM) in the presence or absence of IONPs at a concentration of 2 mg/mL. Data are expressed as mean percentage (%) of control ± SD of three independent experiments with six replicates each.