Table 1.
Size and lipid content of liposomes co-loading IONPs and Cuphen before and after short centrifugation cycles.
| DMPC:CHEMS:DSPE-PEG (57:38:5) |
Ø (nm) |
PDI | Lipid (µmol) (%) |
|---|---|---|---|
| Before centrifugation | 163 ± 2 | 0.06 ± 0.02 | 10 (100%) |
|
Centrifugation
15,000 g, 30 min |
176 ± 2 | 0.06 ± 0.02 | 5.8 ± 0.1 (59%) |
|
Ultracentrifugation
42,000 g, 20 min |
175 ± 1 | 0.08 ± 0.01 | 5.6 ± 0.2 (54%) |
| Values are expressed as average ± SD of at least two independent experiments. DMPC: dimiristoyl phosphatidyl choline; CHEMS: cholesteryl hemisuccinate; DSPE-PEG: distearoyl phosphatidylethanolamine covalently linked to poly(ethylene) glycol 2000; PDI: polidispersity index. Ø: mean size of liposomes. | |||