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. 2020 Apr 6;10(4):693. doi: 10.3390/nano10040693

Table 1.

Size and lipid content of liposomes co-loading IONPs and Cuphen before and after short centrifugation cycles.

DMPC:CHEMS:DSPE-PEG
(57:38:5)		 Ø
(nm)		 PDI Lipid (µmol)
(%)
Before centrifugation 163 ± 2 0.06 ± 0.02 10
(100%)
Centrifugation
15,000 g, 30 min 		176 ± 2 0.06 ± 0.02 5.8 ± 0.1
(59%)
Ultracentrifugation
42,000 g, 20 min 		175 ± 1 0.08 ± 0.01 5.6 ± 0.2
(54%)
Values are expressed as average ± SD of at least two independent experiments. DMPC: dimiristoyl phosphatidyl choline; CHEMS: cholesteryl hemisuccinate; DSPE-PEG: distearoyl phosphatidylethanolamine covalently linked to poly(ethylene) glycol 2000; PDI: polidispersity index. Ø: mean size of liposomes.