. 2020 Apr 21;25(8):1933. doi: 10.3390/molecules25081933

Table 5.

Inverse solvent isotope and solvent viscosity effects for NAD-malic enzyme ¹.

Substrate	Fixed Substrate, Cofactor(s)	SKIE		Solvent Viscosity Effect ²
Substrate	Fixed Substrate, Cofactor(s)	^D2O(k_cat/K_m)	^D2O k _cat	k_cat/K_m	k _cat
malate	NAD⁺, Mg²⁺	0.5 ± 0.1	0.76 ± 0.02	0.6 ± 0.2	0.85 ± 0.07
	NAD⁺, Cd²⁺	0.37 ± 0.06	1.0 ± 0.2	0.61 ± 0.02	0.86 ± 0.08
	NAD⁺, Mn²⁺	0.5 ± 0.3	1.272 ± 0.006	0.62 ± 0.05	1.0 ± 0.1
	NAD⁺, Mg²⁺, fumarate	0.47 ± 0.07	0.80 ± 0.02	0.46 ± 0.03	0.84 ± 0.01
	APAD, ³ Mg²⁺	0.49 ± 0.06	0.91 ± 0.02	0.62 ± 0.06	0.82 ± 0.06
	APAD, Mn²⁺	0.76 ± 0.03	0.99 ± 0.01	0.57 ± 0.01	0.78 ± 0.04
	APAD, Mn²⁺, fumarate	0.81 ± 0.03	1.07 ± 0.02	0.68 ± 0.02	0.85 ± 0.03
NAD⁺	malate, Mg²⁺	0.57 ± 0.02	0.81 ± 0.06	0.689 ± 0.001	0.891 ± 0.001
pyruvate	NADH, CO₂, Mg²⁺	0.42 ± 0.01	0.35 ± 0.08	0.41 ± 0.02	0.44 ± 0.07

¹ Data taken from Karsten et al. [50]. ² Relative solvent viscosity using 9% glycerol as a viscosogen, expressed as the ratio of rate constants, (η_rel = 1)/(η_rel = 1.24). ³ APAD = 3-acetylpryidine adenine dinucleotide.