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. 2020 Apr 15;25(8):1837. doi: 10.3390/molecules25081837

Figure 2.

Figure 2

(A) Determination of safe doses of MNCM extract in fly larvae, the third-instar larvae were fed with MNCM extract (150 µg/mL–1 mg/mL). The surviving flies were counted within 5 days after the first eclosion and the percentage of the survival rate was calculated. (B) Effects of MNCM extract on the accumulation of Aβ1–42 peptides in fly brains. Flies were treated with MNCM extract (150–500 µg/mL) for 28 days, and after that fly heads were lysed and subjected for ELISA. (C) Effects of MNCM extract (150–500 µg/mL) on the locomotory coordination (climbing) of AD flies after 28 days of treatment. (D) Effects of MNCM extract on the BACE-1 activity in fly brains. Flies were treated with MNCM extract (150–500 µg/mL) for 28 days, then fly heads were lysed and subjected for BACE-1 activity determination. The data are representative of three replicates and show mean ± standard deviation (SD). The one-way ANOVA followed by Tukey’s test was used to determine the differences between groups. ***, p < 0.001.