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. 2020 Apr 15;25(8):1837. doi: 10.3390/molecules25081837

Table 1.

Effects of different percentages of aqueous ethanol on MNCM extraction regarding AChE inhibition.

Independent Variable
(Solvents, % (v/v) Aqueous Ethanol)		 Dependent Variable
% AChE Inhibition		 Controlled Variables
0% (v/v) (ultrapure water) 40.29 ± 1.27 a

  • Extraction temperature 30 °C

  • Shaking time 30 min

  • Extraction concentration 30 mg/mL
20% (v/v) 27.07 ± 1.36 b
40% (v/v) ND *
60% (v/v) ND *
80% (v/v) ND *
100% (v/v) (absolute ethanol) ND *

Values expressed are mean ± standard deviation (SD) of triplicate experiments (n = 3). Lower letter case indicates significant differences in each column at p < 0.05 calculated by one-way analysis of variance (ANOVA) and Duncan’s multiple comparison test. * ND = not detected.