Table 2.
Matrix effect and extraction recovery of ECN.
| Nominal Concentration (ng/mL) | Matrix Effect (%) a | Extraction Recovery (%) b |
|---|---|---|
| ECN | ||
| 10.0 | 83.3 ± 7.65 | 62.6 ± 4.56 |
| 25.0 | 86.7 ± 4.28 | 58.5 ± 3.88 |
| 3750 | 89.6 ± 1.50 | 73.4 ± 1.41 |
| 7500 | 93.1 ± 0.86 | 77.7 ± 5.03 |
| Losartan (IS) | ||
| 80.0 | 70.3 ± 1.10 | 99.5 ± 0.93 |
a Matrix effect (%) = ((peak area of analyte spiked after extraction)/(peak area of analyte in neat solution)) × 100. b Extraction recovery (%) = ((peak area of analyte spiked before extraction)/(peak area of analyte spiked after extraction)) × 100. Data are presented as mean ± SD (n = 4).