Table 1.

Sequences of siRNAs and calculated IC₅₀ values for MDR1-GFP gene silencing after transfection into KB-8-5-MDR1-GFP cells by Lipofectamine 2000.

Designation	Sequence 1	IC50, nM
siRNA	5′-GGCUUGACAAGUUGUAUAUGG-3′ 3′-AACCGAACUGUUCAACAUAUA-5′	3.8 ± 3.5
Ch-siRNA	Ch-5′-GGCUUGACAAGUUGUAUAUGG-3′  3′-AACCGAACUGUUCAACAUAUA-5′	29 ± 3
Ch-siSCRm	Ch-5′-CAAGUCUCGUAUGUAGUGGUU-3′  3′-UUGUUCAGAGCAUACAUCACC-5′
TsiRNA-1	5′-GGCUUGACAAGUUGUAUAUGGGGCUUGACAAGUUGUAUAUGGGGCUUGACAAGUUGUAUAUGG-3′ 3′-AACCGAACUGUUCAACAUAUAAACCGAACUGUUCAACAUAUAAACCGAACUGUUCAACAUAUA-5′	0.65 ± 0.14
Ch-TsiRNA-1	Ch-5′-GGCUUGACAAGUUGUAUAUGGGGCUUGACAAGUUGUAUAUGGGGCUUGACAAGUUGUAUAUGG-3′  3′-AACCGAACUGUUCAACAUAUAAACCGAACUGUUCAACAUAUAAACCGAACUGUUCAACAUAUA-5′	16 ± 10
TsiRNA-2	5′-CAGAGGCCGCUGUUCGUUUGAGCGCGAGGUCGGGAUGGAUUUGGCUUGACAAGUUGUAUAUGG-3′ 3′-UCGUCUCCGGCGACAAGCAAACUCGCGCUCCAGCCCUACCUAAACCGAACUGUUCAACAUAUA	13 ± 7
Ch-TsiRNA-2	Ch-5′-CAGAGGCCGCUGUUCGUUUGAGCGCGAGGUCGGGAUGGAUUUGGCUUGACAAGUUGUAUAUGG-3′  3′-UCGUCUCCGGCGACAAGCAAACUCGCGCUCCAGCCCUACCUAAACCGAACUGUUCAACAUAUA–5′	87 ± 13

¹ Ch—cholesterol conjugated via aminohexyl linker; 2′-O-methyl-modified nucleotides are highlighted in bold and underlined.