Table 1.
Kinetics and site-selectivity of RNA cleavage by conjugates 3–20.
| Conjugate | t 1/2 | n − 2 | n − 1 | n | n + 1 | n + 2 | n + 3 |
|---|---|---|---|---|---|---|---|
| 3 1,3 | 14 ± 0.9 h | 1.4% | 2.5% | 89.0% | 7.1% | ||
| 4 1,3 | 3.5 ± 0.4 h | 1.6% | 5.8% | 83.9% | 8.6% | ||
| 13 1 | 13.3 ± 0.8 h | 1.5% | 65.2% | 30.2% | 3.1% | ||
| 14 1 | 6.4 ± 0.2 h | 1.2% | 75.3% | 22.1% | 1.4% | ||
| 15 1 | 6.5 ± 0.5 h | 1.5% | 88.3% | 10.1% | |||
| 16 1 | 5.6 ± 0.1 h | 1.2% | 74.9% | 22.1% | 1.7% | ||
| 5 2,3 | 15.0 ± 1.7 h | 29.0% | 22.7% | 35.8% | 5.1% | 3.6% | |
| 6 2,3 | 10.0 ± 0.3 h | 6.4% | 35.0% | 45.9% | 6.9% | 4.5% | 1.3% |
| 17 2 | 18.3 ± 1.2 h | 5.2% | 4.1% | 52.1% | 22.5% | 9.6% | 2.3% |
| 18 2 | 14.2 ± 0.7 h | 2.4% | 67.4% | 21.8% | 6.1% | 2.2% | |
| 19 2 | 13.0 ± 1.5 h | 10.7% | 71.3% | 9.8% | 6.4% | 1.8% | |
| 20 2 | 9.8 ± 1.0 h | 3.2% | 72.4% | 17.2% | 3.4% | 1.1% |
Determined by peak integration of all cleavage products. n means cleavage between nucleotides 15 and 16, directly after the duplex region. 1 Reaction with RNA 1. 2 Reaction with RNA 2. 3 Taken from reference 39.