Figure 3.

The viability rate analysis of 5-FU combined with allicin compared to each single-agent treatment IC₅₀ dose. The comparison of the co-treatment and individual compounds indicated that the co-treatment was more effective against tumor cells compared to the cytotoxic drug and allicin alone. Abbreviations: NS, not significant; Control, untreated group; 5-FU IC50, group treated with 5-FU at IC₅₀ dose; 5-FU 1/2 IC50, group treated with half of 5-FU IC₅₀ dose; Allicin IC50, group treated with allicin IC₅₀ dose; Allicin 1/2 IC50, group treated with half of allicin IC₅₀ dose; 5-FU 1/2 IC50 + Allicin 1/2 IC50, group treated with the combination of 5-FU and allicin half IC₅₀ doses. The results with p < 0.05 were considered statistically significant (* p < 0.05 and ** p < 0.01).