Table A1.
Samples and results after 16 h at 37 °C.
| 20 wt% BSA (μL) |
EtOH (μL) |
H2O (μL) |
w% BSA |
w% EtOH |
x BSA 10−5 |
x EtOH |
EtOH AA |
Result |
|---|---|---|---|---|---|---|---|---|
| 200 | 1 | 0 | 19.92 | 0.39 | 6.8 | 0.002 | 0.05 | - |
| 200 | 2 | 0 | 19.84 | 0.78 | 6.79 | 0.004 | 0.1 | - |
| 200 | 5 | 0 | 19.61 | 1.93 | 6.75 | 0.01 | 0.24 | - |
| 200 | 10 | 0 | 19.24 | 3.8 | 6.69 | 0.02 | 0.48 | - |
| 200 | 15 | 0 | 18.88 | 5.59 | 6.63 | 0.03 | 0.73 | - |
| 200 | 20 | 0 | 18.54 | 7.31 | 6.56 | 0.04 | 0.97 | - |
| 200 | 25 | 0 | 18.2 | 8.98 | 6.5 | 0.05 | 1.21 | - |
| 200 | 32 | 0 | 17.76 | 11.21 | 6.42 | 0.06 | 1.55 | - |
| 200 | 33 | 0 | 17.7 | 11.52 | 6.41 | 0.06 | 1.6 | - |
| 200 | 34 | 0 | 17.63 | 11.83 | 6.4 | 0.06 | 1.65 | - |
| 200 | 35 | 0 | 17.57 | 12.13 | 6.39 | 0.06 | 1.7 | - |
| 200 | 36 | 0 | 17.51 | 12.44 | 6.38 | 0.06 | 1.74 | - |
| 200 | 37 | 0 | 17.45 | 12.74 | 6.36 | 0.07 | 1.79 | h. v. |
| 200 | 38 | 0 | 17.39 | 13.04 | 6.35 | 0.07 | 1.84 | gel |
| 200 | 39 | 0 | 17.33 | 13.33 | 6.34 | 0.07 | 1.89 | gel |
| 200 | 40 | 0 | 17.27 | 13.63 | 6.33 | 0.07 | 1.94 | gel |
| 200 | 45 | 0 | 16.98 | 15.08 | 6.27 | 0.08 | 2.18 | gel |
| 200 | 50 | 0 | 16.7 | 16.48 | 6.22 | 0.09 | 2.42 | gel |
| 200 | 134.8 | 6.2 | 13.06 | 34.03 | 5.25 | 0.2 | 6.53 | gel |
| 200 | 96.5 | 8.6 | 14.49 | 26.74 | 5.5 | 0.15 | 4.68 | gel |
| 200 | 101.1 | 16.6 | 14.3 | 26.92 | 5.25 | 0.15 | 4.9 | gel |
| 200 | 61.5 | 10.7 | 16.1 | 18.72 | 5.75 | 0.1 | 2.98 | gel |
| 200 | 64.3 | 18.5 | 15.95 | 18.84 | 5.5 | 0.1 | 3.12 | gel |
| 200 | 67.4 | 27 | 15.8 | 18.98 | 5.25 | 0.1 | 3.27 | gel |
| 200 | 28.3 | 5.8 | 17.99 | 9.79 | 6.25 | 0.05 | 1.37 | - |
| 200 | 29.4 | 12.7 | 17.92 | 9.83 | 6 | 0.05 | 1.43 | - |
| 200 | 30.7 | 20.2 | 17.84 | 9.91 | 5.75 | 0.05 | 1.49 | - |
| 200 | 32.2 | 28.4 | 17.75 | 10.01 | 5.5 | 0.05 | 1.56 | - |
| 200 | 33.7 | 37.4 | 17.65 | 10.07 | 5.25 | 0.05 | 1.63 | - |
| 200 | 40.2 | 2.1 | 17.26 | 13.57 | 6.25 | 0.07 | 1.95 | gel |
| 200 | 41.9 | 8.89 | 17.16 | 13.66 | 6 | 0.07 | 2.03 | gel |
| 200 | 43.7 | 16.24 | 17.06 | 13.75 | 5.75 | 0.07 | 2.12 | h. v. |
| 200 | 45.7 | 24.3 | 16.95 | 13.85 | 5.5 | 0.07 | 2.22 | h. v. |
| 200 | 47.9 | 33 | 16.82 | 13.96 | 5.25 | 0.07 | 2.32 | h. v. |
| 200 | 38.5 | 2.66 | 17.36 | 13.04 | 6.25 | 0.07 | 1.87 | gel |
| 200 | 40.1 | 9.44 | 17.27 | 13.12 | 6 | 0.07 | 1.94 | gel |
| 200 | 41.9 | 16.8 | 17.16 | 13.23 | 5.75 | 0.07 | 2.03 | gel |
| 200 | 43.8 | 24.8 | 17.05 | 13.32 | 5.5 | 0.07 | 2.12 | h. v. |
| 200 | 45.8 | 33.7 | 16.94 | 13.39 | 5.25 | 0.07 | 2.22 | - |
| 200 | 36.8 | 3.19 | 17.46 | 12.5 | 6.25 | 0.06 | 1.78 | gel |
| 200 | 38.3 | 9.98 | 17.37 | 12.58 | 6 | 0.06 | 1.86 | h. v. |
| 200 | 40 | 17.4 | 17.27 | 12.68 | 5.75 | 0.06 | 1.94 | - |
| 200 | 41.8 | 25.4 | 17.17 | 12.76 | 5.5 | 0.06 | 2.03 | - |
| 200 | 43.8 | 34.3 | 17.05 | 12.85 | 5.25 | 0.06 | 2.12 | - |
| 200 | 40.6 | 17.2 | 17.24 | 12.85 | 5.75 | 0.07 | 1.97 | gel |
| 200 | 41.2 | 17 | 17.2 | 13.03 | 5.75 | 0.07 | 2 | gel |
| 200 | 50.3 | 42.7 | 16.69 | 14.05 | 5 | 0.07 | 2.44 | gel |
| 200 | 47.2 | 33.2 | 16.86 | 13.77 | 5.25 | 0.07 | 2.29 | gel |
| 200 | 46.5 | 33.4 | 16.9 | 13.58 | 5.25 | 0.07 | 2.25 | - |
| 200 * | 28.5 | 0 | 3.48 | 10.11 | 4.58 | 0.05 | 1.95 | - |
| 200 * | 29.3 | 0 | 13.45 | 10.36 | 4.57 | 0.05 | 2.01 | - |
| 200 * | 30 | 0 | 13.41 | 10.58 | 4.56 | 0.05 | 2.05 | - |
| 200 * | 33.5 | 0 | 13.25 | 11.67 | 4.54 | 0.06 | 2.29 | - |
| 200 ** | 136.1 | 17.8 | 15.38 | 33.02 | 6.5 | 0.19 | 5.04 | gel |
| 200 ** | 141.6 | 24.5 | 14.87 | 33.23 | 6.25 | 0.19 | 5.25 | gel |
| 200 ** | 147.5 | 31.8 | 14.36 | 33.42 | 6 | 0.19 | 5.48 | gel |
| 200 ** | 153.9 | 39.7 | 13.85 | 33.62 | 5.75 | 0.19 | 5.73 | gel |
| 200 ** | 160.9 | 48.3 | 13.32 | 33.83 | 5.5 | 0.19 | 6 | gel |
| 200 ** | 102 | 28.3 | 16.19 | 26.06 | 6.5 | 0.14 | 3.78 | gel |
| 200 ** | 106.2 | 35.4 | 15.66 | 26.25 | 6.25 | 0.14 | 3.94 | gel |
| 200 ** | 110.6 | 43.1 | 15.13 | 26.41 | 6 | 0.14 | 4.11 | gel |
| 200 ** | 115.4 | 51.6 | 14.59 | 26.57 | 5.75 | 0.14 | 4.29 | gel |
| 200 ** | 68 | 38.8 | 17.1 | 18.35 | 6.5 | 0.1 | 2.52 | gel |
| 200 ** | 70.8 | 46.4 | 16.54 | 18.48 | 6.25 | 0.1 | 2.63 | gel |
| 200 ** | 73.7 | 54.5 | 15.99 | 18.6 | 6 | 0.1 | 2.74 | gel |
| 200 ** | 34 | 49.3 | 18.11 | 9.72 | 6.5 | 0.05 | 1.26 | - |
| 200 ** | 46.3 | 45.5 | 17.73 | 12.95 | 6.5 | 0.06 | 1.71 | gel |
| 200 ** | 44.2 | 46.1 | 17.8 | 12.41 | 6.5 | 0.06 | 1.64 | gel |
| 90 | 84 | 110 | 6.76 | 24.89 | 2.36 | 0.12 | 9.05 | h. v. |
| 80 | 84 | 120 | 6.01 | 24.89 | 2.08 | 0.12 | 10.18 | - |
| 70 | 84 | 130 | 5.26 | 24.89 | 1.8 | 0.12 | 11.63 | - |
| 100 | 100 | 85 | 7.58 | 29.9 | 2.79 | 0.16 | 9.69 | gel |
| 100 | 115 | 70 | 7.67 | 34.8 | 2.94 | 0.19 | 11.15 | gel |
| 100 | 130 | 55 | 7.76 | 39.82 | 3.12 | 0.23 | 12.6 | gel |
| 100 | 162.2 | 51.8 | 7.15 | 45.74 | 3 | 0.28 | 15.72 | gel |
| 100 | 191.6 | 42.7 | 6.81 | 51.44 | 3 | 0.32 | 18.57 | t. g. |
| 100 | 206 | 38.2 | 6.65 | 54.05 | 3 | 0.35 | 19.97 | t. g. |
| 100 | 221 | 33.6 | 6.49 | 56.62 | 3 | 0.37 | 21.42 | t. g. |
| 100 | 235 | 29.1 | 6.36 | 58.95 | 3 | 0.4 | 22.78 | t. l. |
| 100 | 250 | 24.5 | 6.22 | 61.31 | 3 | 0.42 | 24.24 | t. l. |
| 100 | 265 | 20 | 6.08 | 63.54 | 3 | 0.45 | 25.69 | t. l. |
| 100 | 280 | 15.4 | 5.95 | 65.69 | 3 | 0.47 | 27.14 | t. l. |
| 100 | 294 | 10.9 | 5.83 | 67.66 | 3 | 0.5 | 28.5 | t. g. |
| 100 | 79 | 90.4 | 7.91 | 24.66 | 2.8 | 0.13 | 7.66 | gel |
| 100 | 85.1 | 103.6 | 7.39 | 24.8 | 2.6 | 0.13 | 8.25 | gel |
| 100 | 92.2 | 118.9 | 6.86 | 24.94 | 2.4 | 0.13 | 8.94 | - |
| 100 | 295 | 14.04 | 5.77 | 67.12 | 2.95 | 0.49 | 28.6 | t. g. |
| 100 | 305 | 10.9 | 5.69 | 68.45 | 2.95 | 0.51 | 29.57 | t. g. |
| 100 | 285 | 9.79 | 5.98 | 67.19 | 3.07 | 0.49 | 27.63 | t. g. |
| 100 | 289 | 12.72 | 5.87 | 66.92 | 3 | 0.49 | 28.02 | t. g. |
| 100 | 300 | 9.09 | 5.78 | 68.45 | 3 | 0.51 | 29.08 | t. g. |
| 100 | 182 | 0 | 8.21 | 58.95 | 4.00 | 0.413 | 17.67 | - |
| 100 | 173 | 3 | 8.35 | 56.99 | 4.00 | 0.392 | 16.80 | - |
| 100 | 160 | 7 | 8.57 | 54.12 | 4.00 | 0.362 | 15.53 | - |
| 100 | 150 | 10 | 8.76 | 51.83 | 4.00 | 0.340 | 14.56 | - |
| 100 | 140 | 13 | 8.95 | 49.43 | 4.00 | 0.317 | 13.59 | t. g. |
| 100 | 121 | 19 | 9.33 | 44.51 | 4.00 | 0.274 | 11.75 | gel |
| 100 | 92 | 28 | 9.97 | 36.19 | 4.00 | 0.208 | 8.93 | gel |
| 100 | 76 | 33 | 10.36 | 31.08 | 4.00 | 0.172 | 7.38 | gel |
| 100 | 50 | 41 | 11.08 | 21.86 | 4.00 | 0.113 | 4.85 | gel |
| 100 | 30 | 47 | 11.72 | 13.87 | 4.00 | 0.068 | 2.91 | - |
| 100 | 94 | 0 | 11.48 | 42.58 | 5.00 | 0.266 | 9.13 | gel |
| 100 | 81 | 4 | 11.91 | 38.06 | 5.00 | 0.229 | 7.86 | gel |
| 100 | 68 | 8 | 12.37 | 33.19 | 5.00 | 0.193 | 6.60 | gel |
| 100 | 49 | 14 | 13.10 | 25.32 | 5.00 | 0.139 | 4.76 | gel |
| 100 | 36 | 18 | 13.66 | 19.40 | 5.00 | 0.102 | 3.50 | gel |
| 100 | 360 | 20 | 4.95 | 70.30 | 2.58 | 0.526 | 34.95 | - |
| 100 | 270 | 14 | 6.12 | 65.14 | 3.08 | 0.470 | 26.21 | - |
| 100 | 240 | 0 | 6.91 | 65.44 | 3.54 | 0.481 | 23.30 | t. g. |
| 100 | 200 | 12 | 7.41 | 58.49 | 3.55 | 0.402 | 19.42 | - |
| 100 | 170 | 21 | 7.84 | 52.57 | 3.55 | 0.342 | 16.50 | - |
| 100 | 140 | 31 | 8.28 | 45.75 | 3.54 | 0.280 | 13.59 | t. g. |
| 100 | 100 | 43 | 9.01 | 35.56 | 3.54 | 0.201 | 9.71 | gel |
| 100 | 70 | 52 | 9.65 | 26.65 | 3.55 | 0.141 | 6.80 | gel |
| 100 | 40 | 62 | 10.33 | 16.31 | 3.53 | 0.080 | 3.88 | gel |
| 100 | 200 | 131 | 5.14 | 40.59 | 2.00 | 0.226 | 19.42 | - |
| 100 | 169 | 141 | 5.34 | 35.62 | 2.00 | 0.191 | 16.41 | - |
| 100 | 140 | 150 | 5.55 | 30.64 | 2.00 | 0.158 | 13.59 | - |
| 100 | 100 | 162 | 5.87 | 23.14 | 2.00 | 0.113 | 9.71 | - |
| 100 | 60 | 100 | 8.09 | 19.14 | 2.75 | 0.093 | 5.83 | gel |
| 100 | 400 | 100 | 3.88 | 61.21 | 1.80 | 0.407 | 38.84 | - |
| 100 | 400 | 50 | 4.30 | 67.78 | 2.15 | 0.487 | 38.84 | - |
| 100 | 50 | 400 | 3.71 | 7.31 | 1.10 | 0.031 | 4.85 | - |
| 150 | 200 | 10 | 9.44 | 49.65 | 4.27 | 0.322 | 12.95 | t. g. |
| 150 | 180 | 16 | 9.74 | 46.11 | 4.27 | 0.290 | 11.65 | t. g. |
| 150 | 160 | 22 | 10.06 | 42.33 | 4.27 | 0.258 | 10.36 | gel |
| 150 | 140 | 28 | 10.40 | 38.29 | 4.28 | 0.226 | 9.06 | gel |
| 150 | 100 | 41 | 11.12 | 29.23 | 4.26 | 0.161 | 6.47 | gel |
| 150 | 83 | 46 | 11.47 | 25.04 | 4.27 | 0.134 | 5.37 | gel |
| 150 | 70 | 50 | 11.75 | 21.64 | 4.27 | 0.113 | 4.53 | gel |
| 150 | 60 | 53 | 11.98 | 18.91 | 4.27 | 0.097 | 3.88 | gel |
| 150 | 50 | 56 | 12.22 | 16.07 | 4.27 | 0.081 | 3.24 | gel |
| 150 | 38 | 60 | 12.50 | 12.49 | 4.27 | 0.061 | 2.46 | - |
* 25 w% stock solution was used. ** 15 % stock solution was used. h.v. refers to high viscosity solution; t.l. refers to turbid liquid (no gel, not viscous, but due to denaturation of proteins the solution is turbid); t.g. refers to turbid gel. - means no gel has been observed.