Table 1.
Characteristics of the method for the TMAO and the TMA determination by LC/MS/MS.
| Characteristic | TMA | TMAO | ||
|---|---|---|---|---|
| LLOD | 0.26 µM | 0.57 µM | ||
| LLOQ | 0.40 µM | 5.0 µM | ||
| ULOQ | >50 µM | >10 mM | ||
| Accuracy (%) | 0.5 µM | 96.2 | 10 µM | 98.4 |
| 2 µM | 97.0 | 50 µM | 102.8 | |
| 5 µM | 101.1 | 100 µM | 102.1 | |
| 10 µM | 102.0 | 250 µM | 102.1 | |
| 20 µM | 102.3 | 500 µM | 100.6 | |
| 40 µM | 101.3 | 2000 µM | 101.3 | |
Lower limit of detection (LLOD) and Lower limit of quantitation (LLOQ) were estimated by Calibration Approach as described in methods. Upper limit of quantitation (ULOQ) = greater than highest standard investigated. Accuracy = ratio of the TMA and TMAO concentrations measured to the TMA and TMAO concentrations added to water followed by the same procedure as the urine samples for the TMAO and TMA measurement, respectively.