Figure 5.

Intra-signalling pathway of platelet activation PCSK9-mediated: p38MAP Kinase, p47^phox and PLA₂ phosphorylation. p38 phosphorylation, p47^phox phosphorylation and cPLA2 phosphorylation was analysed in platelets incubated with PCSK9 (1–2 ng/mL) and stimulated with subthreshold concentration (STC) of collagen (0.25 µg/mL) (A–C) in presence or less of anti-CD36, anti-LOX1, anti-PCSK9 (A–C) and incubated with or without SB (A), Nox2ds-tat (B) and AACOCF3 (C) (n = 3 independent experiments; *p < 0.05 **p < 0.001 in quantitative analysis). A representative Western blot of p38, p47phox and cPLA2 phosphorylation (B–C).